Summary
The Series 2000 Cold Light Fluorescence Detector CLF1 & CLF2
uses flow through sampling and results are outputted to the Microstream
data processor via an analogue output socket. The fluorimeters
are fixed wavelength instruments using a high brightness excitation
source and emition cutoff filters. The detection limit for quinine
sultphate is 0.1ppb. 32 Fluorescence methods for automatic analysis
have been developed and one particularly for low level Aluminium
in clinical determination. New manual methods can be adapted.
Product Details
The Series 2000 Cold Light Fluorescence Detector is an important
addition to the range of analytical instruments for continuous
flow analysis, flow injection and process control analysis. The
introduction of this detector compliments and extends the capabilities
of Burkard continuous flow technology where levels of sensitivity
can now be significantly improved. Its application in areas of
pharmaceutical quality assurance, routine environmental analysis,
beverage testing procedures, medical science and others has demonstrated
improved performance by up to a factor of 10.
Operation
The CLF1 & CLF2 use flow through sampling and results are
outputted to the Microstream data processor via an analogue output
socket. The fluorimeter has been constructed as a fixed wavelength
instrument using twin high brightness light sources for excitation.
Solid state ultraviolet (UV) excitation offers increased energy,
lower cost and longer life compared to traditional bulky (UV)
light sources. The absence of heat avoids drift during operation
and as the bandwidth of these devices (15-20 nm) is similar to
interference filters an excitation filter is not required. A
lower cost cut-off filter is supplied for the emission light
output, which is detected by silicon photo-diode. Wavelength
change involves the replacement of the fixed pre-positioned light
source and cut-off filter of the appropriate wavelength.
Applications
Standard flow cells of various volumes are available for fluorescence
applications or when the instrument is used as a nephelometer
for measuring turbidity in water samples. With some electronic
change the instrument can be configured for luminometry measuring
low light levels from bioluminescence. The setup for nephelometry
is identical to that for fluorimetry. A heated flowcell assembly
is provided for enzymatic determinations. The detection limit
for quinine sultphate is 1 ppb.
32 Fluorescence methods for automatic analysis
have been developed and one particularly for low level Aluminium
in clinical determination. New manual methods can be adapted.
The following list is just a few of the compounds that have been
analysed by using fluorescence, either in flow analysis systems
or by chromatography. For compounds not on this list please contact
us.
DNA
Aluminium
Albumin
Lactate
Boron
Bile acids
Mercury
Hydrogen peroxide
Cerium(IV)
Urea
Galactosidase |
Histidine
Phenylalanine
Gallium
IgG
Copper
Pyridoxal
Manganese
Ochratoxin A
Vitamin B1
Quinine
Rhodamine G |
Histamine
Glycine Glucose
Terbium
Cyanide
Pyridoxal-5-phosphate
Iron Cerium(III)
Cyclophosphamide
Phosphate |
Instrumentation
In addition to the Series 2000 Fluorimeter, Burkard
can supply a range of autosamplers, pumps, injection valves,
chemistry manifolds and data acquisition systems to provide a
completely automatic system for analysis by fluorescence.
All systems are supplied with fully developed methods.
Installation on site is provided together with full training
in the technique and in the use of the Data System. Burkard Scientific
would also be pleased to carry out method development and to
assist in the validation of the method and instrumentation.
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